Capillary Electrophoresis: – Introduction Principle Component procedure Advantages Introduction: – Capillary electrophoresis is a technique used in analytical chemistry to separate ions based on their electrophoretic mobility in a buffer-filled capillary under the influence of an electric field. It’s widely…
Electrophoresis:- Introduction Principle Requirement Procedure Types 1. Electrophoresis: – The term electrophoresis describes the migration of change particles under the influence of an electric field. 2. Principle: – Many important biological molecules such as amino acids, peptides, proteonucleotides and nucleic…
RADIOIMMUNOASSAY Introduction: – Used to determine the concentrations of antigen present in the blood serum of the patient. Principle:- It is best on competitive binding between the Rio levels antigen (hot antigen) and unlebeled antigen (cold Antigen) with selected antibody…
Determination of Urobilinogen Principle: – Urobilinogen reacts with para dimethyl amino benzaldehyde in an acidic medium (in Ehrlich reagent) to form pink colored compound appearance of pink color indicate the presence of urobilinogen in Urine. Method: – Ehrlich’s method Requirement:…
Determination of bile salt Bile:- it is a yellowish color fluid that is secreted by the liver and stored in the gall bladder. Method:- Hay’s Method Principle: – Bile salt when present on the surface tension of urine when sulfur…
Determination of bile pigment in urine Principle:- When Barium Chloride reagent is added to urine it combines with sulfate radicals in urine and a precipitate of Barium sulfate is formed if bile pigment is present in the urine then it…
occult blood in urine The chemical method detects free hemoglobin and myoglobin. occult blood in urine Principle of occult blood in urine:- The peroxidase activity of hemoglobin in urine decomposes hydrogen peroxide and oxidized benzidin to form a green-blue color…
Ketone body determination in urine Method :- Rothera test Principle:- Nitroprusside used for this test reacts with both acetone and acetoacetic acid in the presence of alkali to produce a purpled Coloured.. –Requirement:- Test tube pipette Test babe back. Liquor…
determination of glucose in urine Name of the test – Benedicts, qualitative Test principle of the test:- 5ml of a qualitative reagent is heated with an eighth drop (0.5ml) of urine, glucose present in urine reduces the Cupric ion’s presence…
Serological and Immunological techniques used in the diagnosis of Microbial Infection. Enzyme-Linked Immunosorbent Assay (ELISA):- The term ELISA was introduced by Engvall and Perlmann in 1971 to describe a subset of the widely used immunoassay technique. It is a highly…